PBT and vPvB refer to substances with "Persistent, Bioaccumulative and Toxic" or "very Persistent and very Bioaccumulative" properties.
The assessment of PBT and vPvB properties is part of the chemical safety assessment required under the REACH Regulation. The chemical safety assessment shall be conducted for substances that are subjects to registration and manufactured or imported into EU in quantities of 10 tonnes or more per year, and for substances that are subjects to authorization.
ISO and GLP compliance
DHI performs ecotoxicological tests and QSARs aiming at the screening as well as the definitive criteria for P, vP, B, vB and T (see list below). At our ecotoxicological laboratory we perform tests in accordance with ISO 17025, accredited by DANAK (the Danish Accreditation and Metrology Fund) and in compliance with the OECD Principles of Good Laboratory Practice (GLP).
| Persistence (P and vP) |
Test description |
Guideline |
Ready biodegradability |
OECD 301 |
Enhanced ready biodegradability |
Modified OECD 301 |
Inherent biodegradability |
Zahn-Wellens OECD 302B MITI II test OECD 302C |
Biowin 2/ 3/ 6 (QSAR) |
EPI suite (US-EPA) |
Aerobic and anaerobic transformation in soil |
OECD 307 |
Aerobic and anaerobic transformation in aquatic sediment systems |
OECD 308 |
Simulation test – aerobic mineralization in surface water |
OECD 309 |
Bioaccumulation (B and vB) |
Test description |
Guideline |
Log Pow (QSAR) |
EPI suite (US-EPA) |
Log Pow |
OECD 117 |
Bioaccumulation in fish |
OECD 305 |
Toxicity (T) |
Test description |
Guideline |
Short-term aquatic toxicity |
OECD 201, 202, 203 |
Long-term, chronic aquatic toxicity |
OECD 210, 211 |